TBLASTX 2.0.10 [Aug-26-1999]

 

 

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,

Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),

"Gapped BLAST and PSI-BLAST: a new generation of protein database search

programs",  Nucleic Acids Res. 25:3389-3402.

 

Query= gnl|UG|Hs#S305372 Human mRNA for KIAA0174 gene, complete cds

/cds=(63,1157) /gb=D79996 /gi=1136407 /ug=Hs.75824 /len=2348

         (2348 letters)

 

Database: na_clot.dros

           13,830 sequences; 9,510,273 total letters

 

 

 

                                                                   Score     E

Sequences producing significant alignments:                        (bits)  Value

 

 

>904_1 EST composition (unordered) :

           LD14015.5prime,LP10908.5prime,HL02273.5prime,

           SD10104.5prime,LD04979.5prime,LD06861.5prime,

           LD31316.5prime,HL01580.5prime

           Length = 732

          

 Score = 51.9 bits (107), Expect = 5e-007

 Identities = 28/80 (35%), Positives = 41/80 (51%)

 Frame = +3 / +1

 

                                                                      

Query: 96  KSEFEISHKSP*TIGEKENGTGPESKEGDC*LSGCWER*TSSDPCGAHYPGRLPRGGHGD 275

           + +  I  +SP   GE+E G   + +EG+  L G  +     D  GAH+ G L  GG+G+

Sbjct: 148 EDQSSIGSQSPEIAGEEEGGADAKVQEGNSRLFGHGQNGKGEDSRGAHH*GGLLGGGNGN 327

 

                              

Query: 276 PGAVL*PAAGSVWPYPVYEG 335

            G VL P A  + P+   EG

Sbjct: 328 GGDVLRPVARQIRPHHPDEG 387

 

 

 Score = 77.6 bits (163), Expect = 9e-015

 Identities = 33/72 (45%), Positives = 41/72 (56%)

 Frame = +2 / +3

 

                                                                      

Query: 122 IALNYWRKRKRNWPRKQGRRLLTIWLLGKMNELGSVWSTLSGKTTSWRPWRSWSCTVTCC 301

           IA N WR+R+R+  +  GR+  TIW   K    G  WST  G+TT WR W+ W CT TCC

Sbjct: 174 IA*NCWRRRRRS*RKSPGRK*PTIWPRAKRKGRGFAWSTSLGRTTWWRQWKWWRCTATCC 353

 

                      

Query: 302 WLGLALSSL*RN 337

               A S  *R+

Sbjct: 354 SPDSASSPR*RS 389

 

 

 Score =  180 bits (387), Expect(2) = 7e-062

 Identities = 73/105 (69%), Positives = 89/105 (84%)

 Frame = +1 / +2

 

                                                                      

Query: 163 QKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELD 342

           QK+RKEIADYLA GK ERARIRVEHIIREDYLVEAME++E+YCDLLLARFGLI  MKELD

Sbjct: 215 QKSRKEIADYLATGKTERARIRVEHIIREDYLVEAMEMVEMYCDLLLARFGLITQMKELD 394

 

                                                       

Query: 343 SGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRT 477

           +G+AE V++L+W  PRLQS++AELKI++D    KY  ++ +  RT

Sbjct: 395 TGIAEPVASLVWVCPRLQSDIAELKIISDIFVTKYGPQFAEQSRT 529

 

 

 Score = 75.7 bits (159), Expect(2) = 7e-062

 Identities = 29/46 (63%), Positives = 37/46 (80%)

 Frame = +1 / +2

 

                                                        

Query: 493 VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPP 630

           V+++LMHKL+++APPK+LVE YLI IAKNYN+ YEPD   M E  P

Sbjct: 548 VSEKLMHKLTLQAPPKLLVENYLIAIAKNYNIEYEPDPQCMQEDQP 685

 

 

 Score = 43.7 bits (89), Expect(2) = 6e-015

 Identities = 22/46 (47%), Positives = 31/46 (66%)

 Frame = -1 / -1

 

                                                        

Query: 629 GGASAMTTESGS*GTL*FFAISIRYLSTRILGGASTLSLCISLSFT 492

           G +S +   SGS   L*F A+++R  ST+ LGGA ++SLC+S S T

Sbjct: 684 G*SSCIHCGSGSYSML*FLAMAMR*FSTKSLGGACSVSLCMSFSDT 547

 

 

 Score = 54.7 bits (113), Expect(2) = 6e-015

 Identities = 32/73 (43%), Positives = 38/73 (51%)

 Frame = -1 / -1

 

                                                                      

Query: 446 YLAQS*SATIFNSATSDWSRGAAQINVDTDSARPESSSFIDWIRPNXXXXXXXXXXXXXX 267

           YL  + S  IF+SA S   RG  Q  + T SA P SSSFI  +RPN             

Sbjct: 498 YLVTNMSDMIFSSAISLCRRGHTQTKLATGSAIPVSSSFIWVMRPNLASNRSQYISTISI 319

 

                       

Query: 266 XSTR*SSRIMCST 228

            ST+*SS +MCST

Sbjct: 318 ASTK*SSLMMCST 280

 

 

 Score = 46.4 bits (95), Expect(2) = 4e-022

 Identities = 19/36 (52%), Positives = 23/36 (63%)

 Frame = -3 / -3

 

                                              

Query: 615 HDHRVRFIGYVVILCNFNQVSLHQDFGWGFHTQLVH 508

           H  R+RF+  VV+L N N+V LHQ  GW    QLVH

Sbjct: 670 HTLRIRFVFDVVVLGNGNEVILHQKLGWCLQRQLVH 563

 

 

 Score = 76.2 bits (160), Expect(2) = 4e-022

 Identities = 35/92 (38%), Positives = 50/92 (54%)

 Frame = -3 / -3

 

                                                                       

Query: 447 ILGTELISNYFQLSHF*LESRSSPDQCRHRFSQTRI*FLHRLDKAKPSQQQVTVQLQDLH 268

           + G + +    QL +  L + + P+Q  HR S   +  LH  D+A+  +QQV V L   H

Sbjct: 499 VFGHKYVRYDLQLGYIAL*AGTHPNQAGHRLSDPCVQLLHLGDEAESGEQQVAVHLHHFH 320

 

                                          

Query: 267 GLHEVVFPDNVLHTDPSSFIFPSSQIVSNLLP 172

            LH+VV P++VLH +P  F F   QIV   LP

Sbjct: 319 CLHQVVLPNDVLHANPRPFRFARGQIVGYFLP 224

 

 

  Database: na_clot.dros

    Posted date:  Nov 24, 1999 11:08 AM

  Number of letters in database: 9,510,273

  Number of sequences in database:  13,830

 

Lambda     K      H

   0.318    0.135    0.401

 

 

Matrix: BLOSUM62

Number of Hits to DB: 62014374

Number of Sequences: 13830

Number of extensions: 1107411

Number of successful extensions: 54282

Number of sequences better than 1.0e-003: 1

length of query: 782

length of database: 3,170,091

effective HSP length: 48

effective length of query: 734

effective length of database: 2,506,251

effective search space: 1839588234

effective search space used: 1839588234

frameshift window, decay const: 50,  0.1

T: 13

A: 40

X1: 16 ( 7.3 bits)

X2: 0 ( 0.0 bits)

S1: 41 (21.7 bits)

S2: 83 (40.9 bits)