TBLASTX 2.0.10 [Aug-26-1999]

 

 

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,

Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),

"Gapped BLAST and PSI-BLAST: a new generation of protein database search

programs",  Nucleic Acids Res. 25:3389-3402.

 

Query= gnl|UG|Hs#S998743 Homo sapiens mRNA for KIAA0560 protein,

complete cds /cds=(406,4671) /gb=AB011132 /gi=3043643 /ug=Hs.129952

/len=5915

         (5915 letters)

 

Database: na_clot.dros

           13,830 sequences; 9,510,273 total letters

 

 

 

                                                                   Score     E

Sequences producing significant alignments:                        (bits)  Value

 

>5892_1 EST composition (unordered) : GM03621.5prime,LP02069.5prime

            Length = 967

           

 Score =  104 bits (222), Expect(3) = e-131

 Identities = 43/51 (84%), Positives = 46/51 (89%)

 Frame = +2 / +1

 

                                                              

Query: 1610 LLRNFNLFRLESTYEIRQDIEDSVSRMKPWQSEYGGVVFGGWARMAQPIVA 1762

            LLRNFNLFRLESTYEIRQDIED+VSRM PW+SE G VVFGGWARMA P+ A

Sbjct: 1    LLRNFNLFRLESTYEIRQDIEDAVSRMLPWKSEDGDVVFGGWARMALPLPA 153

 

 

 Score =  220 bits (475), Expect(3) = e-131

 Identities = 87/125 (69%), Positives = 104/125 (82%)

 Frame = +2 / +3

 

                                                                        

Query: 1754 IVAFTVVEVAKPNIGENWPTRVRADVTINLNVRDHIKDEWEGLRKHDVCFLITVRPTKPY 1933

            I +F VVEVAKP++GE  P+RVRADV + L+VR  IK EWE LRKHDVCFLITV+PT+PY

Sbjct: 144  IASFAVVEVAKPHLGEKKPSRVRADVGVTLSVRREIKAEWENLRKHDVCFLITVKPTQPY 323

 

                                                                       

Query: 1934 GTKFDRRRPFIEQVGLVYVRGCEIQGMLDDKGRVIEDGPEPRPNLRGESRTFRVFLDPNQ 2113

            GTK+  R PF+ QVGLV VRGCE++GMLD  GRVIEDGPEPRP L+GE+R +RV+LD NQ

Sbjct: 324  GTKYKHREPFVPQVGLVSVRGCEVEGMLDANGRVIEDGPEPRPQLQGENRCYRVWLDSNQ 503

 

                

Query: 2114 YQQDM 2128

            Y+ DM

Sbjct: 504  YRLDM 518

 

 

 Score =  184 bits (396), Expect(3) = e-131

 Identities = 70/96 (72%), Positives = 84/96 (86%)

 Frame = +2 / +3

 

                                                                       

Query: 2138 IQNGAEDVYETFNIIMRRKPKENNFKAVLETIRNLMNTDCVVPDWLHDIILGYGDPSSAH 2317

            +Q GA+DVYE+FNI+MRRKPKENNFKAVLETIR+LMNT+CVVP WLHDI+LGYGDP +AH

Sbjct: 525  LQEGADDVYESFNILMRRKPKENNFKAVLETIRHLMNTECVVPPWLHDILLGYGDPGAAH 704

 

                                               

Query: 2318 YSKMPNQIATLDFNDTFLSIEHLKASFPGHNVKVTV 2425

            YS MPNQ  +L+FNDTF+   HL+ASFP +N+K  V

Sbjct: 705  YSNMPNQERSLEFNDTFMDYNHLEASFPQYNLKCEV 812

 

 

 Score = 38.2 bits (77), Expect(3) = 3e-013

 Identities = 20/41 (48%), Positives = 24/41 (57%)

 Frame = +3 / +2

 

                                                    

Query: 1620 TLTSSA*NQLMKFVRTLKIVSAE*SHGNLNMAV*CLVVGRE 1742

            TLT SA N  MK  +  K+ SA   HGN  MA+ C V G+E

Sbjct: 11   TLTYSAWNPPMKSAKISKMPSAACCHGNPKMAMLCSVAGQE 133

 

 

 Score = 35.4 bits (71), Expect(3) = 3e-013

 Identities = 30/88 (34%), Positives = 38/88 (43%)

 Frame = +1 / +2

 

                                                                        

Query: 1753 HCGFHCR*SGQTQHR*KLANPSSCRCYHKSQCQRSHQR*MGRSS*A*CMLFNYRTSHKTL 1932

            HC      SGQT    K A  S+C C   S      Q  +G  S A  +L + R ++ T+

Sbjct: 143  HCQLCRCRSGQTSSWRKEALQSTC*CGRNSFGAPRDQGRVGEPSKARRVLPHNRKANATI 322

 

                                        

Query: 1933 WH*V*PEETFY*AGWPGLCQRL*NSGHA 2016

             H*V     F   G  G C  +*  GHA

Sbjct: 323  RH*VQAPRAFCSPGRLGQCPWV*GGGHA 406

 

 

 Score = 39.6 bits (80), Expect(3) = 3e-013

 Identities = 20/54 (37%), Positives = 29/54 (53%)

 Frame = +3 / +1

 

                                                                 

Query: 2136 LYKMEQRMCMKLLI***GENQRKITLRLCWRLFGT**ILIVWYLTGCTISF*VM 2297

            +Y+ E  M  ++L      + R+IT R CW+ +  **IL  W+  G T SF  M

Sbjct: 523  IYRREPTMSTRVLTSSCAASPRRITSRPCWKPYVI**ILNAWFRLGFTTSFWAM 684

 

 

 Score = 39.1 bits (79), Expect(3) = 2e-018

 Identities = 20/41 (48%), Positives = 23/41 (55%)

 Frame = +1 / +3

 

                                                     

Query: 1642 INL*NSSGH*R*CQQNEAMAI*IWRCSVWWLGANGPAHCGF 1764

            I+L*N   + R  Q + AM I  WRC V WLG NG A   F

Sbjct: 33   IHL*NPPRYRRCRQPHVAMEIRRWRCCVRWLGKNGSAIASF 155

 

 

 Score = 45.0 bits (92), Expect(3) = 2e-018

 Identities = 27/74 (36%), Positives = 37/74 (49%)

 Frame = +3 / +1

 

                                                                       

Query: 1800 KTGQPEFVQMLP*ISMSEITSKMNGKVFVSMMYAF*LPYVPQNLMALSLTGGDLLLSRLA 1979

            K   PE+V M  *+      S+ +G+ F S   A   P   +N  ALS +   LL  R A

Sbjct: 190  KRSPPEYVLMWA*LFRCAERSRQSGRTFESTTCASS*P*SQRNHTALSTSTASLLFPR*A 369

 

                         

Query: 1980 WFMSEAVKFRACWM 2021

            W +S  V++RAC M

Sbjct: 370  WSVSVGVRWRAC*M 411

 

 

 Score = 46.9 bits (96), Expect(3) = 2e-018

 Identities = 27/66 (40%), Positives = 41/66 (61%)

 Frame = +1 / +2

 

                                                                       

Query: 2140 TKWSRGCV*NF*YNNEEKTKGK*L*GCAGDYSEPDEY*LCGT*LAARYHFRLWGPK*CTL 2319

            T  SR C+  F*+ +  + +G+*L G AG+++  +EY*+ G+ LA+R+ F LW    C L

Sbjct: 527  TGGSRRCLREF*HPHAPQAQGE*LQGRAGNHTSFNEY*MRGSALASRHPFGLW*SWSCPL 706

 

                 

Query: 2320 FENAQS 2337

               AQS

Sbjct: 707  Q*YAQS 724

 

 

 Score = 62.0 bits (129), Expect(3) = 4e-038

 Identities = 29/90 (32%), Positives = 51/90 (56%)

 Frame = -2 / -3

 

                                                                       

Query: 2410 IMTREAGF*MLNGEKCIIEIKGGNLIGHFRIMCTTWVPIT*NDIVQPVRYHTISIHQVPN 2231

            I+  E G  M+   + I++++   LIGH  ++ ++ + I   D+V+P R H  SIH + 

Sbjct: 797  IILGETGLKMIVIHEGIVKLQTAFLIGHITVVGSSRITIAQKDVVKPRRNHAFSIH*MTY 618

 

                                         

Query: 2230 SLQHSLKVIFLWFSPHYYIKSFIHILCSIL 2141

              QH L+VI L  + H  +K+ + I+ S+L

Sbjct: 617  GFQHGLEVILLGLAAHEDVKTLVDIVGSLL 528

 

 

 Score = 89.5 bits (189), Expect(3) = 4e-038

 Identities = 43/128 (33%), Positives = 75/128 (58%)

 Frame = -2 / -3

 

                                                                       

Query: 2134 IGHILLILVWIQKHSKCP*FSSKIGSGFRSIFNDTSFIIQHALNFTASDINQANLLNKRS 1955

            I HI  +L+ +Q ++     + ++   FR++F D++  I HAL+ T +D +QA L NKR

Sbjct: 524  IVHIQAVLIGVQPNAVATILTLQLWPWFRAVFYDSAIGI*HALHLTPTDTDQAYLGNKRL 345

 

                                                                        

Query: 1954 PPVKLSAIRFCGTYGN*KAYIMLTKTFPFIFDVISDIEIYGNICTNSGWPVFTYVGFGHF 1775

              + LSA+     YG  +A+++L+K  P   D+ +  + Y +I T SG  +F  + FGHF

Sbjct: 344  AVLVLSAVWLRWLYGYEEAHVVLSKVLPLCLDLSAHRKSYAHISTYSGGLLFAKMRFGHF 165

 

                   

Query: 1774 NDSESHNG 1751

            ++ ++ NG

Sbjct: 164  DNGKAGNG 141

 

 

 Score = 46.9 bits (96), Expect(3) = 4e-038

 Identities = 25/48 (52%), Positives = 26/48 (54%)

 Frame = -3 / -3

 

                                                           

Query: 1764 KATMGWAIRAQPPNTTPPYSDCHGFILLTLXXXXXXXXXXXSKRKRLK 1621

            KA  G AI AQPPNTT P SD HG + LT            SKR RLK

Sbjct: 155  KAGNGRAILAQPPNTTSPSSDFHGNMRLTASSISWRIS*VDSKRNRLK 12

 

 

 Score = 76.2 bits (160), Expect(3) = 4e-058

 Identities = 46/87 (52%), Positives = 52/87 (58%)

 Frame = -3 / -1

 

                                                                       

Query: 2400 GKLAFKCSMERNVSLKSRVAI*LGIFE*CALLGSP*PKMISCSQSGTTQSVFIRFRIVSS 2221

            GKLA +      VSL SR+  *LGI  * A  GSP*PK +S SQ GTT SVFI+ R+VSS

Sbjct: 787  GKLASR*L*SMKVSLNSRLRS*LGILL*WAAPGSP*PKRMS*SQGGTTHSVFIK*RMVSS 608

 

                                      

Query: 2220 TAXXXXXXXXXXXXXLKVSYTSSAPFC 2140

            TA             LK+S TSSAP C

Sbjct: 607  TALKLFSLGLRRMRMLKLS*TSSAPSC 527

 

 

 Score =  132 bits (282), Expect(3) = 4e-058

 Identities = 66/108 (61%), Positives = 72/108 (66%)

 Frame = -3 / -1

 

                                                                       

Query: 2076 SPLRLGLGSGPSSMTRPLSSSMP*ISQPLT*TRPTCSIKGLLRSNLVP*GFVGRTVIKKH 1897

            SP   GLGSGPSSMTRPL+SSMP  S P T T+PT   KG     LVP G VG TV++KH

Sbjct: 466  SPCSCGLGSGPSSMTRPLASSMPSTSHPRTLTKPTWGTKGSRCLYLVPYGCVGFTVMRKH 287

 

                                                            

Query: 1896 TSCLRRPSHSSLM*SLTLRFMVTSARTRVGQFSPMLGLATSTTVKATM 1753

            TSC RR SHS+L+   T R   TSART  G FSP  GLATSTT K  M

Sbjct: 286  TSCFRRFSHSALISRRTERVTPTSARTLEGFFSPR*GLATSTTAKLAM 143

 

 

 Score = 57.4 bits (119), Expect(3) = 4e-058

 Identities = 25/45 (55%), Positives = 31/45 (68%)

 Frame = -2 / -2

 

                                                        

Query: 1744 HSRPTTKHYTAIFRLPWLHSADTIFNVLTNFIS*F*AEEVKVP*Q 1610

            HS P T+H  AIF  PW H+AD IF++L +FI  F AE VKV *+

Sbjct: 135  HSCPATEHNIAIFGFPWQHAADGIFDILADFIGGFQAE*VKVA*E 1

 

 

 Score = 33.6 bits (67), Expect(3) = 1e-010

 Identities = 18/51 (35%), Positives = 23/51 (44%)

 Frame = -1 / -2

 

                                                              

Query: 2024 YHPACPEFHSL*HKPGQPAQ*KVSSGQT*CHKVLWDVR*LKSIHHAYEDLP 1872

            +H ACP  H+  H P  P + K     T*C  V   +R   S   A+E  P

Sbjct: 414  WHLACPPPHTHGH*PSLPGEQKARGACT*CRMVALALRL*GSTRRAFEGSP 262

 

 

 Score = 29.0 bits (57), Expect(3) = 1e-010

 Identities = 13/32 (40%), Positives = 16/32 (49%)

 Frame = -1 / -2

 

                                           

Query: 1829 HLHELGLASFHLCWVWPLQRQ*KPQWAGPFAP 1734

            H H L  ASF    VWPL+++   QW     P

Sbjct: 219  HQHVLWRASFRQDEVWPLRQRQSWQWQSHSCP 124

 

 

 Score = 41.4 bits (84), Expect(3) = 1e-010

 Identities = 16/35 (45%), Positives = 25/35 (70%)

 Frame = -1 / -1

 

                                              

Query: 1751 AGPFAPNHQTLHRHIQIAMASFC*HYLQCPDEFHK 1647

            A PF P+H+T HRH++I+MA+  * +L+    FH+

Sbjct: 142  AEPFLPSHRTQHRHLRISMATCG*RHLRYLGGFHR 38

 

 

  Database: na_clot.dros

    Posted date:  Nov 24, 1999 11:08 AM

  Number of letters in database: 9,510,273

  Number of sequences in database:  13,830

 

Lambda     K      H

   0.318    0.135    0.401

 

 

Matrix: BLOSUM62

Number of Hits to DB: 139921163

Number of Sequences: 13830

Number of extensions: 2156503

Number of successful extensions: 85113

Number of sequences better than 1.0e-003: 1

length of query: 1971

length of database: 3,170,091

effective HSP length: 50

effective length of query: 1921

effective length of database: 2,478,591

effective search space: 4761373311

effective search space used: 4761373311

frameshift window, decay const: 50,  0.1

T: 13

A: 40

X1: 16 ( 7.3 bits)

X2: 0 ( 0.0 bits)

S1: 41 (21.7 bits)

S2: 86 (42.3 bits)