TBLASTX 2.0.10 [Aug-26-1999]

 

 

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,

Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),

"Gapped BLAST and PSI-BLAST: a new generation of protein database search

programs",  Nucleic Acids Res. 25:3389-3402.

 

Query= gnl|UG|Hs#S1367619 Homo sapiens mRNA for KIAA0859 protein,

complete cds /cds=(102,1973) /gb=AB020666 /gi=4240206 /ug=Hs.23159

/len=4356

         (4356 letters)

 

Database: na_clot.dros

           13,830 sequences; 9,510,273 total letters

 

 

 

                                                                   Score     E

Sequences producing significant alignments:                        (bits)  Value

 

2839_1 EST composition (unordered) : LP04144.5prime,LD12777.5prime   253  3e-067

 

>2839_1 EST composition (unordered) : LP04144.5prime,LD12777.5prime

           Length = 681

          

 Score = 32.2 bits (64), Expect(2) = 5e-007

 Identities = 21/52 (40%), Positives = 26/52 (49%)

 Frame = +3 / +2

 

                                                              

Query: 96  *EHEPLT*KFQGVWLR*LLGEVLPAARKESFRVVWNLPGTVRGAT*IYQAQG 251

           * HE      +GV    LL  +L  AR E  R+V  +PG VR    I+QA G

Sbjct: 56  *NHEFTAQNPRGVRTDRLLERILQEAR*EGLRMVRRIPGVVRPDPQIHQAGG 211

 

 

 Score = 40.0 bits (81), Expect(2) = 5e-007

 Identities = 20/56 (35%), Positives = 31/56 (54%)

 Frame = +3 / +2

 

                                                                  

Query: 312 LSGYSEHRHQ*GCHQANEGM*CHPTAPDELLEDGHDADGVS*CLVPGGVGQGHPGC 479

           +SGY +HR+     + N G  C   A +E+  +G D++ V    + G VGQG+ GC

Sbjct: 272 ISGYHKHRYISHRCEENAGTECQIAAGNEVSANGRDSNDVPG*KLFGVVGQGNAGC 439

 

 

 Score =  253 bits (546), Expect = 3e-067

 Identities = 99/183 (54%), Positives = 130/183 (70%)

 Frame = +1 / +3

 

                                                                       

Query: 103 MNLLPKSSREFGSVDYWEKFFQQRGKKAFEWYGTYLELCGVLHKYIKPREKVLVIGCGNS 282

           MNLLPK+  EF   DYW +FF++RG+KAFEWYG YLELC  +HKYIKP +++L++GCGNS

Sbjct: 63  MNLLPKTREEFAQTDYWNEFFKKRGEKAFEWYGEYLELCDQIHKYIKPADRILMLGCGNS 242

 

                                                                       

Query: 283 ELSEQLYDVGYRDIVNIDISEVVIKQMKECNATRRPQMSFLKMDMTQMEFPDASFQVVLD 462

           +LS  +YD G+RDI NIDIS + +K+M E NA  RP+M FL+MD T M FPD SF V LD

Sbjct: 243 KLSMDMYDTGFRDITNIDISPIAVKKMLELNAKSRPEMKFLQMDATAMTFPDESFSVSLD 422

 

                                                                      

Query: 463 KGTLDAVLTDEEEKTLQQVDRMLAEVGRVLQVGGRYLCISLAQAHILKKAVGHFSREGWM 642

           KGTLDA+  D+E +T   V+    E+ R ++ GGRY+ ISL Q HIL   +    +   M

Sbjct: 423 KGTLDALFADDEPETRAVVENYFKEILRTMRNGGRYVGISLLQEHILNFLLDFLPKHNCM 602

 

             

Query: 643 VRV 651

           +R+

Sbjct: 603 LRI 611

 

 

 Score = 24.0 bits (46), Expect(2) = 5e-032

 Identities = 9/15 (60%), Positives = 11/15 (73%)

 Frame = -1 / -2

 

                          

Query: 606 LQDVSLSQGDAEIAT 562

           ++DV L QGD  IAT

Sbjct: 566 IEDVLLQQGDTHIAT 522

 

 

 Score =  133 bits (284), Expect(2) = 5e-032

 Identities = 72/150 (48%), Positives = 82/150 (54%)

 Frame = -2 / -3

 

                                                                       

Query: 521 STCCKVFSSSSVRTASRVPLSNTTWNEASGNSICVMSIFKKLIWGRRVALHSFICLMTTS 342

           ST   V  SSS   AS VPLSN T   +SGN I V SI +  I GR +A  S I     

Sbjct: 481 STTALVSGSSSANRASSVPLSNDTEKLSSGNVIAVASICRNFISGRDLAFSSSIFFTAMG 302

 

                                                                      

Query: 341 LMSMFTISR*PTSYSCSLSSELPHPITSTFSLGLIYLCSTPHSSR*VPYHSKAFFPRCWK 162

            +SMF ISR P SY   L+ ELPHP     S GL+YL    H+SR  PYHSKAF PR  K

Sbjct: 301 DISMFVISRNPVSYISMLNLELPHPSIKILSAGLMYLWIWSHNSRYSPYHSKAFSPRFLK 122

 

                                        

Query: 161 NFSQ*STEPNSLELLGKRFMFLLPKLRFQD 72

           N  Q*S   NS  +LG +FM      R Q+

Sbjct: 121 NSFQ*SVCANSSRVLGSKFMVSTTTKRMQN 32

 

 

 Score = 77.6 bits (163), Expect = 2e-014

 Identities = 42/132 (31%), Positives = 65/132 (48%)

 Frame = -1 / -2

 

                                                                      

Query: 498 FLICQDSIQGALVQHHLERGIRKLHLRHVHLQEAHLGPSGGITFLHLLDDNLTDVDVHYI 319

           F++ + SIQ +LVQ H +  IR+ H   VHLQ+ H  P  GI F H L  +   + V  I

Sbjct: 458 FVVGKQSIQRSLVQRHRKAFIRERHCCRVHLQKLHFRPRFGIQFQHFLHSDGRYIYVCDI 279

 

                                                                      

Query: 318 PIAHIIQLLTQF*VATPNHQHLFPGLDIFM*HPAQFQVGSIPXXXXXXXXXXXXLPIVNG 139

           P + ++ +  QF V    HQ+    LD+F+   AQ QV ++P            +P++ 

Sbjct: 278 PKSCVVHIHAQFGVTASQHQNPIRRLDVFVDLVAQLQVFAVPFEGLLTALLEEFVPVIGL 99

 

                      

Query: 138 AKLPGTFR*EVH 103

            +L   F   +H

Sbjct: 98  CELLAGFGQ*IH 63

 

 

  Database: na_clot.dros

    Posted date:  Nov 24, 1999 11:08 AM

  Number of letters in database: 9,510,273

  Number of sequences in database:  13,830

 

Lambda     K      H

   0.318    0.135    0.401

 

 

Matrix: BLOSUM62

Number of Hits to DB: 119667667

Number of Sequences: 13830

Number of extensions: 2195036

Number of successful extensions: 109224

Number of sequences better than 1.0e-003: 1

length of query: 1452

length of database: 3,170,091

effective HSP length: 49

effective length of query: 1402

effective length of database: 2,492,421

effective search space: 3494374242

effective search space used: 3494374242

frameshift window, decay const: 50,  0.1

T: 13

A: 40

X1: 16 ( 7.3 bits)

X2: 0 ( 0.0 bits)

S1: 41 (21.7 bits)

S2: 85 (41.8 bits)